`Hitler Became Chancellor in January 1933 Because He Was...

`Hitler became Chancellor in January 1933 because he was leader of the most popular party in Germany. ` How far do you agree with this judgement? The Nazi party was slowly making its ascent into the whirlwind world of politics; at first glance they appeared to be making no progress, due to this they were paid very little attention. The government’s negligence of the party allowed them to grow gradually and undetected. Although their popularity contributed to Hitler`s appointment as Chancellor, it can be argued that there were more dominant factors that assisted Hitler in his rise to power. The prematurity of the government is often seen as the underlying factor; however some historians argue that the actions of Hindenburg, Von Papen and†¦show more content†¦The depression caused a fear of communist uprising, but the Nazis very quickly swooped in and diffused the ‘communist bomb’. The parties popularity prior to the depression was not great, but once the depression became more apparent, so did there power. Hitler was able to take advantage of a country that was emotionally, politically and socially unstable. Besides the depression the Nazi use of fear tactics was another reason as to why they gained so many supporters. The people were afraid of the SA and their enforcement of violence upon anybody who dared oppose the German ideology. The depression, combined with fear assisted Hitler in his appointment to chancellor in 1933. For many Germans, Hitler was the light at the end of the tunnel, his policies appeared to be in the best interest of the people. He gave the people something to hold on to, something to believe in. his followers always felt as if they were part of something. He illustrated himself as being the only hope the country had, ``unsere letter hoffnung``. The nazis had successfully portrayed themselves as the only party that appealed to the nation, the people had no other choice but to turn to extremism. They no longer had a trustable government and the popularity of the Nazi`s appealed to them more than a turbulent government did. Although the government had made its own promises to the people, its previous blunders made them untrustworthy in the eyes of the Germans. The signing of the treaty of

A Arranged Marriage - 1992 Words

Humans, regardless of their location in the world have many aspects of their lives that are similar to one another. From daily life to traditions humans share a lot of attributes with one another in which they do not realize. One of the greatest parts of life would be finding a person out in the world that you are privileged to spend the rest of your life with. An equal half to you, someone who bares all the grueling and breath taking moments that life has planned. The practice of marriage has been around since the earliest civilizations. To this day still continues just as strong. However the possibility of finding the potential other person to spend the rest of your life can find you or can be found through an outside connection. As a result of modern social constructs and the methodology of traditional arranged marriages, they do not possess the marital requirements essential to succeed in the western culture. An arranged marriage can be defined as the union of two individuals to be married, brought together by a third party individual outside of the relationship. Throughout the course of history, arranged marriages have had a substantial role in shaping it, originally dating back centuries before and were considered a common practice around the majority of cultures. Marriage, for example in Hinduism is considered a coming together of two souls, and the longevity of it can extend into their next lives. In addition, it is also seen as a dharma meaning a mandatoryShow MoreRelatedThe Marriage Of Arranged Marriage1619 Words   |  7 Pagesexpectations. Should this be the way to go about marriage, or does this hinder a person’s ability to live for themselves? In India alone, around 90% of marriages are arranged by their families (CNN). They would choose who ever they believe would best fit the person, but not necessarily love them (Queano). Although arranged marriage is a custom in many countries, is it the right thing to do? People deserve the right to choose for themselves whom they will marry. Marriage is about love and finding the right personRead MoreThe Marriage Of Arranged Marriage1666 Words   |  7 Pageswife? That is how arranged marriages work, someone else choses your spouse for you. We are now living in a generation where we are highly influenced by what we see in the media. Cultures that practice arranged marriage are now shifting toward the Western norms of marriage. Many people are not familiar with arranged marriages and come to judge to quickly without having any knowledge on the topic. Arranged marriage is a marriage that is organized by two families for the marriage of their son or daughterRead MoreThe Marriage Of Arranged Marriage1895 Words   |  8 Pages Arranged marriage is a form of marital union in which family members or matchmakers select spouses for someone based on cultural background, financial status, dietary habits, religion and other key factors. This type of marriage is mostly practiced in the Middle East, South East Asia, Africa and some South American countries. In contrast, love marriage (free will) is a form of marriage that is used in countries that practice arranged marriages to define a marriage in which partners are allowedRead More Arranged Marriages Essay1105 Words   |  5 Pages Arranged Marriages What is an arranged marriage? Well in the Webster’s dictionary it is defined as a marriage where the marital partners are chosen by others based on considerations other than the pre-existing mutual attraction of the partners. This habit has been very common in noble families, especially in reigning ones, at the scope of combining and perhaps enforcing the respective strengths of originary families (and kingdoms) of the spouses. A relevant part of history has been influencedRead MoreArranged Marriages And Love Marriages1617 Words   |  7 PagesEnglish 1A 5 May 2015 Arranged Marriage: In the broadest sense, marriages have been divided into two general categories; arranged marriages and love marriages. As the name implies, an arranged marriage is defined as a marriage where the bride and groom are exclusively selected by a third party (usually their parents), while in a love marriage, people choose their marital partners based on their feelings or attraction for one another. In America and Western Europe, marriages based on love, have dominatedRead MoreArranged Marriage : A Type Of Marriage1216 Words   |  5 PagesThe definition of an Arranged Marriage is; a type of marriage that is established before a lengthy relationship. To make the definition more clear, it is the opposite of a love marriage. Typically in a love marriage, the final step in the relationship is marriage. That is not the case in an arranged marriage. One of the first steps besides meeting your new spouse, is getting married. The main factor of an arranged marriage are the parents. The parents a re what makes the marriage possible because theyRead MoreA Study On Arranged Marriages1715 Words   |  7 PagesProfessor Berger Research Paper Core 7 Arranged Marriages Cultures and countries around the world have differing views on many topics such as whether or not babies should be baptized, vaccinations, abortion and marriages. One topic that has been of controversy and is viewed differently in many countries is marriage and whether or not arranged marriages are better than love marriages. There are several different reasons why people in India believe that arranged marriages are the best; likewise, people inRead MoreEssay on Arranged Marriages786 Words   |  4 Pagescomes to mind when you hear the words arranged marriage? I am sure a happy, respectful, loving relationship was not what popped into your mind. Arrange marriages are a complex subject, and the concept is perceived as a human rights issue where individuals loose the right to freedom of choice. In saying that, my initial opinion of an arranged marriage was not held in high regard. However, after being introduced to someone who is in the process of an arranged marriage; and conductin g my own research; myRead MoreAre Arranged Marriages and Forced Marriages Unethical1550 Words   |  7 PagesAre Arranged Marriages and Forced Marriages Unethical? Would you consider having your marriage forced or arranged to a person you never met or seen before? Most probably no, but this is happening to many young females and males all over the world. Arranged and forced marriages have been a heated debate in certain cultures, justifying whether it is good or bad it’s hard to judge. There have however also been many changes to these traditions in contemporary society, regarding culture clashing. ResearchingRead MoreThe Procedure Of Arranged Marriages2654 Words   |  11 Pagesprocedures in which a person goes through in arranged marriages. We must first understand what arrange marriages is, its procedures and its traditions before we can talk about love marriages in comparison to it. Love marriages are marriages that are done based on love and individuals often know enough about their mate they are getting married to. My main focus in this paper is about arranged marriages in India and what individuals go through in this type of marr iage. Growing up I always knew that when one

Tddc17 †Lab 2 Search Free Essays

TDDC17 - ­? Lab 3 Part 2 Q5 P (Meltdown) = 0,02578 P(Meltdown | Ica weather) = 0. 03472 b)   Suppose that both warning sensors indicate failure. What is the risk of a meltdown in that case? Compare this result with the risk of a melt- ­? down when there is an actual pump failure and water leak. We will write a custom essay sample on Tddc17 – Lab 2 Search or any similar topic only for you Order Now What is the difference? The answers must be expressed as conditional probabilities of the observed variables, P(Meltdown|†¦ ). P(Meltdown | PumpFailureWarning, WaterLeakWarning) = 0,14535 P (Meltdown | PumpFailure, WaterLeak) = 0,2 c)   The conditional robabilities for the stochastic variables are often estimated by repeated experiments or observations. Why is it sometimes very difficult to get accurate numbers for these? What conditional probabilites in the model of the plant do you think are difficult or impossible to estimate? a) What is the risk of melt- ­? down in the power plant during a day if no observations have been made? What if there is icy weather? It is hard to fully understand all possible factors that can effect or trigger an event and how they interact with each other. Observations are always a description of the past and is not always accurate in forecasting the future. E. g. Icy weather is not a thing you can measure and span over a wide range of weather conditions including combinations of precipitation, wind and temperature. d)   Assume that the â€Å"IcyWeather† variable is changed to a more accurate â€Å"Temperature† variable instead (don’t change your model). What are the different alternatives for the domain of this variable? What will happen with the robability distribution of P(WaterLeak | Temperature) in each alternative? The domain decreases in size of possible states as for example precipitation and wind is no longer a part of the estimations. The temperature will be represented as an absolute number or intervals, instead of just true or false. Resulting in a lot more defining of the probabilities of the child nodes with aspect to each value/interval of temperature. Q6 a) What does a probability table in a Bayes ian network represent? The probability table shows the probability for all states of the node given the states of the parent nodes. b)   What is a joint probability distribution? Using the chain rule on the structure of the Bayesian network to rewrite the joint distribution as a product of P(child|parent) expressions, calculate manually the particular entry in the joint distribution of P(Meltdown=F, PumpFailureWarning=F, PumpFailure=F, WaterLeakWaring=F, WaterLeak=F, IcyWeather=F). Is this a common state for the nuclear plant to be in? Kedjeregeln ger foljanade: P(alla ar falska) = P(ICYWEATHER) * P(PUMPFAILURE) * P(PW | PUMPFAILURE) * P(MELTDOWN| PUMPFAILURE, WL) * P(WL | ICYWEATHER) * P(WATERLEAKW | WL) = 0,95 * 0,9 * 0,95 * 1 * 0,9 * 0,95 = 0,69 Ja, detta ar ett vanligt tillstand. c)   What is the probability of a meltdown if you know that there is both a water leak and a pump failure? Would knowing the state of any other variable matter? Explain your reasoning! P(Meltdown | PumpFailure, WaterLeak ) = 0,8. No other variables matter. When all the parents values are observed they alone determine the child value. ) Calculate manually the probability of a meltdown when you happen to know that PumpFailureWarning=F, WaterLeak=F, WaterLeakWarning=F and IcyWeather=F but you are not really sure about a pump failure. P(Meltdown = T | PUMPFAILURE osaker, resten falska )= P(ICYWEATHER) * P(WL | ICYWEATHER) * P(WATERLEAKW | WATERLEAK)* [P(PUMPFAILURE=T) * P(PW | PUMPFAILURE=T) * P(MELTDOWN=T | PUMPFAILURE=T,WL) + P(PUMPFAILURE=F) * P(PW | PUMPFAILUR E=F) * P(MELTDOWN=T | PUMPFAILURE=F,WL)] = 0,95 * 0,9 * 0,95 * (0,1 * 0,1 * 0,16 + 0,9 * 0,95 * 0,01) = 0,008 (1) P(MELTDOWN=F | PUMPFAILURE osaker, resten falska)=P(ICYWEATHER) * P(WL | ICYWEATHER) * P(WATERLEAKW | WL)* [P(PUMPFAILURE=T) * P(PW | PUMPFAILURE=T) * P(MELTDOWN=F | PUMPFAILURE=T,WL) @+ P(PUMPFAILURE=F) * P(PW | PUMPFAILURE=F) * P(MELTDOWN=F | PUMPFAILURE=F,WL)] = 0,95 * 0,9 * 0,95 * (0,1 * 0,1 * 0,84 + 0,9 * 0,95 * 0,99) =0,694 (2) (1) och (2) =; alfa = 1 / (0,008 + 0,69) = 1,42 0,008 * 1,42 = 0,012 0,694 * 1,42 = 0,988 Part 3 During the lunch break, the owner tries to show off for his employees by demonstrating the many features of his car stereo. To everyone’s disappointment, it doesn’t work. How did the owner’s chances of urviving the day change after this observation? Without knowing whether the radio is working or not, the probability of him surviving is 0,99001. If the radio is not working the probability is 0,98116. How   does the bicycle change the owner’s chances of survival? With the bicycle the probability of surviving is 0. 99505. Small inc rease. It   is possible to model any function in propositional logic with Bayesian Networks. What does this fact say about the complexity of exact inference in Bayesian Networks? What alternatives are there to exact inference? Yes but it might be complex and you might sometimes have to add new nodes. For example if you want to model an OR-relationship you have to add a new node with truthtable probabilities that match. An alternative to exat inference is probabilistic indifference. Things might not always be true or false with a predefined probability. With probabilistic inference yuou can reuse a full joint distribution as the â€Å"knowledge base† Part 4 Changes in graph Mr. H-S sleeping ( T = 0. 3, F = 0. 7) Mr HS reacts in a competent way: WaterleakWarn. Pumpfailurewarning Mr HS sleeping T T T T F F F F T T F F T T F F T F T F T F T F T 0. 0 0. 8 0. 0 0. 7 0. 0 0. 7 0. 0 0. 0 P(Survives | Meltdown, Mr HS reacts) incresing 9% (0. 9) The   owner had an idea that instead of employing a safety person, to replace the pump with a better one. Is it possible, in your model, to compensate for the lack of Mr H. S. ‘s expertise with a better pump? Yes, by increasing the probability of the pump not failing with 0. 05. The chance of survival increases to 0. 99713 Mr   H. S. fell asleep on one of the plant’s couches. When he wakes up he hears someone scream: â€Å"There is one or more warning signals beeping in your control room! â€Å". Mr H. S. realizes that he does not have time to fix the error before it is to late (we can assume that he wasn’t in the control room at ll). What is the chance of survival for Mr H. S. if he has a car with the same properties as the owner? (notice that this question involves a disjunction which can not be answered by querying the network as is) Clarification:Maybe something could be added to or modified in the network. By adding a new node called warning, which represents the OR-relationship of WaterLeakWarning and PumpFailureWarning, i. e. Warning is true if WaterLeakWarning is true or if PumpFailureWarning is true or if They are both true and is false if they are both false. P(survives) = 0. 98897 if Warning is observed true. What   unrealistic assumptions o you make when creating a Bayesian Netwo rk model of a person? That a persons actions are predictable and that he never gains more experience as time passes, which would effect the probabilities of his actions. Describe how you would model a more dynamic world where for example the â€Å"IcyWeather† is more likely to be true the next day if it was true the day before. You only have to consider a limited sequence of days. By adding nodes representing the weather of the previous days. E. g. one node representing the day before, one bubble representing the day before that and so on†¦ Tommy Oldeback, tomol475 Emma Ljungberg, emmlj959 How to cite Tddc17 – Lab 2 Search, Essay examples

Phil Lit free essay sample

Course Description The course is a study of literary genres exemplified by the selected literary texts from different regions of the Philippines written at different periods in the Philippine literary history. It aims to equip students with literary appreciation that may guide them towards wholesome development of the innate potentials and values. Credits 3 units Pre-Requisite Introduction to Literature Course Objectives The course has the following objectives: 1. show the relevance of the study of literature in human’s life; 2. link the present to the future based on the thoughts of the past as presented and preserved in the literary texts; 3. point out the value of reading Philippine literary texts; 4. identify and differentiate the various elements of the different genres like short story; 5. articulate the importance of each element in the proper understanding of the genres; 6. showcase the meaning and lessons derived from the literary texts; 7. recognize the value the literary prowess of the writers; 8. We will write a custom essay sample on Phil Lit or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page help students appreciate the richness and value of Philippine literature; and 9. relate literature to human experiences. Learning Competencies Students are expected to: 1. identify the importance of literature to human endeavor; 2. relate to the characters that personify the students’ character or experience; 3. infer the message implied in the different genres; 4. assimilate the lessons in life inherent in the different literary genres; 5. choose the correct paths for self-improvement as inspired by the literary texts; 6. interpret figurative meanings of poetry and identify its elements; 7. possess positive outlook in education through selected essays; 8. read poems observing proper phrasing and expression; 9. analyze the implications of the moves and motives of the characters/persona in the different genres studied; and 10. appreciate and value the richness and imaginative power of man to express ideas/expressions. Instructional Modes/ Learning Activities 1. lecture 2. discussion 3. Pair/group work 4. Debates 5. Role play 6. Film viewing 7. Reporting 8. Storytelling 9. Recital 10. Movie and Book review Content Coverage I. Introduction A. Literature defined B. Importance of Literature C. Philippine Folk Literature D. Literary genres I. The short story A. Short story defined B. Elements of short story C. The Short Stories 1. Death into Manhood 2. How my Brother Leon Brought Home a Wife 3. Dead Stars 4. Faith, Love, Time and Dr. Lazaro 5. Wedding Dance 6. The House on Zapote Street 7. We Filipinos are Mild Drinkers II. Essay A. Essay defined B. Types of Essay C. Essay 1. College Uneducation 2. Sonia 3. Where is the Patis? 4. Male Homosexuality in the Philippines (Garcia) III. Poetry A. Poetry defined B. Elements of Poem C. Representative poems 1. Ink 2. Quietness 3. Like the Molave 4. Eulogy of Roaches IV. Play A. Play defined B. Elements of a Play C. Sepang Loca Learning Resources 1. CD/DVDs 2. Audio-video equipments 3. TV set 4. LCD projector/laptop 5. Textbooks 6. Newspaper/magazines 7. Internet References 1. Balarbar, Corazon V. ,et. al. Gems in Philippine Literature. Manila: National BookStore. 1989. 2. Calixihan, Jovita O. and Lucesa Y. Diano. Gems in Afro-Asian Literature. Manila: National BookStore. 1989. 3. Roseburg, A. G. Pathways to Philippine Literature in English. Phoenix Publishing House. 1996 4. Saymo, Apolinario S. Philippine Literature. Trinitas Publishing, Inc. 2004 5. Tomeldan, Yolanda V. ,et. al. Prism an Introduction to Literature. Manila: National Book Store. 1986 http://en. wikipedia. org www. seasite. niu. edu Course Requirements See www. arcy-tca. wikispaces. com for assignments and updated announcements. Additional requirements: 1. Participation in class activities (dyads, triads, groups) 2. Pass quizzes and long examinations(Midterm and Finals) 4. Perform choral reading/participate in the speech choir inter-class competition 5. Create a Letter to the World as a major project. Course Policies 1. The rule on failing mark for 20% unexcused absences shall be strictly enforced (Chapter 4, Section 2, p. 24 of TCA Student Manual). 2. Short quizzes will be given unannounced; however, schedule for long quizzes shall be announced at least one week prior to its administration. 3. Mid-Term and Final Exams shall be given only to students who have completely or partially settled their accounts for the specified period. 4. Students will be held responsible for all assignments and requirements for the entire content on the course missed regardless of reasons for his absence (Chapter 4, Section 2, p. 24 of TCA Student Manual). 5. Only students officially enrolled in the course will be allowed to attend the class. 6. Talking during examinations, possession of textbooks or notes of any kind (unless authorized), giving or receiving information or any other attempts at communication shall render the offender to disciplinary action (Chapter 5, Section 4, p. 26 of the Student Manual). 7. The professor is not obliged to give a special or late test to any student who fails to take an examination at the regular time, except upon a written request approved by the Dean of the Institute concerned (Chapter 5, Section 5, p. 26 of the Student Manual). 8. Grades of 3. 10-4. 00 are conditional failures, and must be removed by re-examination before the enrolment for the next term (Chapter 6, Section 1, p. 27 of the Student Manual). 9. When given a grade of INC. (Incomplete) , the student shall complete the grade within one year, otherwise, a grade of 5. 0 is automatically given by the registrar. 10. A student under the influence of liquor and/or any dangerous/prohibited drug shall not be allowed entry in the classroom to attend class. 11. All students enrolled in this class is advised to read Chapter 7, pp. 84-93 of the TCA Student Manual for other salient policies and guidelines. Prepared by ARCELYN N. SANGALANG THOMAS FERREBEE Instructor I US Peace Corps Volunteer Recommending Approval EMERSON CUZZAMO ARLENE F. VALDEZ Chair, BEEd Program Coordinator, Academic Program Approved NOEL J. POTERO, Ph. D. MARIA TERESA SJ. VALDEZ, Ph. D Dean, Institute of Education Vice-President, Academic Affairs TCA’s Vision : TCA as Center of Excellence in tertiary education, research, and training for sustainable agro-industrial development TCA’s Mission: Provision of tertiary education geared towards the: pursuit of a better quality of life thru sustainable agriculture food security; provision of knowledge, skills, ideas and values to make students productive, effective and competent in agro-industrial development; acceleration of the development of professionals to provide leadership in various agriculture-based disciplines; search for knowledge to harness the bio-diversity resources of the region. IEd’s Goals : High quality and excellent pre-service and post-graduate teacher education Relevance of pre-service and post-graduate training to national and international standards Effectiveness in conveying knowledge and facilitating teaching-learning processes and activities Efficiency in continuously developing human resource skills, competence, expertise, and leadership for the education sector BEEd Objectives : To produce graduates who: 1. have a competitive edge in meeting the demands of the basic education industry here and abroad 2. will become professional teachers who are efficient and effective facilitators of learning 3. possess genuine appreciation of human ideas and values of refinement, and commitment to the progress of the nation as well as of all humanity; and 4. will become teachers who are proud of their profession and who appreciate Philippine culture and that of the world.

Comical Side of Scoop essays

Comical Side of Scoop essays Evelyn Waugh was one of literature's great curmudgeons and a scathingly funny satirist. Scoop is a comedy of England's newspaper business of the 1930s and the story of William Boot, an innocent hick from the country who writes careful essays about the habits of the badger (Editorial Reviews). "With this book England's wittiest novelist sets a new standard for comic extravaganza...the real message about SCOOP is that it is thoroughly enjoyable, uproariously funny and that everyone should read it at once" (The New York Times). This is just one example of the popularity of the comical relief hidden within the bindings of the book Scoop. This book sets forth a new standard for comic extravaganza. The novel reads as though it had been formed with slapstick, but it is architecture of amazement. For example, his delightful style of the keys of typewriters in the personal quarters of one of Fleet Streets greatest press lords made no more sound than the drumming of a bishops finger tips on an upholstered prie-dieu; the buzzers of the telephones were muffled and purred like wart cats; the massive double doors, encased in New England rosewood, by their weight, polish and depravity of design, proclaimed unmistakably, Nothing but Us stand between you and Lord Copper (Waugh). Other additives were set forth with just the names of the newspapers and of the characters. Newspapers have fabulous names- the Brute, the Beast. A Communist named Pappenhacker, one of the cleverest men in Fleet Street, (Waugh 92) has difficulty finding places to eat because of his bullying of waiters (Waugh 92). This bullying has sound theory behind it. Every time you are polite to a proletarian, Pappenhacker believes, you help bolster up the capitalist system (Gelder 1). Pappenhacker hated the lower class. Another chara ...

10X TBE Electrophoresis Buffer Protocol or Recipe

10X TBE Electrophoresis Buffer Protocol or Recipe TBE and TAE are used as buffers in molecular biology, primarily for electrophoresis of nucleic acids.  Tris buffers are used under slightly basic pH conditions, as for DNA electrophoresis, because this keeps the DNA soluble in the solution and deprotonated so it will be attracted to the positive electrode and will migrate through a gel. EDTA is an ingredient in the solution because this common chelating agent protects nucleic acids from degradation by enzymes. The EDTA chelates divalent cations that are cofactors for nucleases that may contaminate the sample. However, since the magnesium cation is a cofactor for DNA polymerase and restriction enzymes, the concentration of EDTA is kept purposely low (around 1 mM concentration). 10X TBE Electrophoresis Buffer Materials 108 g of Tris base [tris(hydroxymethyl)aminomethane]55 g of boric acid7.5 g of EDTA, disodium saltDeionized water Preparation for  the 10X TBE Electrophoresis Buffer Dissolve the Tris, boric acid, and EDTA in 800 ml of deionized water.​Dilute the buffer to 1 L. Undissolved white clumps may be made to dissolve by placing the bottle of solution in a hot water bath. A magnetic stir bar can aid the process. You do not need to sterilize the solution. Although precipitation may occur after a span of time, the stock solution is still usable. You can adjust the pH using a pH meter and dropwise addition of concentrated hydrochloric acid (HCl). Its fine to store TBE buffer at room temperature, although you may wish to filter the stock solution through a 0.22-micron filter to remove particle that would foster precipitation. 10X TBE Electrophoresis Buffer Storage Store the bottle of 10X buffer solution at room temperature. Refrigeration will accelerate precipitation. Using 10X TBE Electrophoresis Buffer The solution is diluted before use. Dilute 100 mL of 10X stock to 1 L with deionized water. 5X TBE Stock Solution Recipe The advantage of the 5X solution is that its less likely to precipitate. 54 g of Tris base (Trizma)27.5 grams of boric acid20 mL of 0.5 M EDTA solutionDeionized water Preparation Dissolve the Tris base and boric acid in the EDTA solution.Adjust the pH of the solution to 8.3 using concentrated HCl.Dilute the solution with deionized water to make 1 liter of 5X stock solution. The solution may also be diluted to 1X or 0.5X for electrophoresis. Using a 5X or 10X stock solution by accident will give you poor results because as much heat will be generated.  In addition to giving you poor resolution, the sample may be damaged. 0.5X TBA Buffer Recipe 5X TBE stock solutionDistilled deionized water Preparation Add 100 mL of the 5X TBE solution to 900 mL of distilled deionized water. Mix thoroughly before use. Limitations Although TBE and TAE are common electrophoresis buffers, there are  other options  for low-molarity conductive solutions, including lithium borate buffer and sodium borate buffer. The problem with TBE and TAE are that Tris-based buffers limit the electric field that can be used in electrophoresis because too much charge causes a runaway temperature.

Mitosis Lab Report Example | Topics and Well Written Essays - 1250 words

Mitosis - Lab Report Example The prepared and preserved slides were observed under the microscope. The different stages of the mitosis such as interphase, prophase, meta phase, anaphase and telophase of the cell along with the cytokinesis were identified and the stages were differentiated. Introduction: All the living things are made of cells. In a multi-cellular organism, two types of cell division occur: they are mitosis and meiosis. Mitosis or Somatic cell division is the process in which one cell divides into two equal cells with genetic identity. The cell division is necessary for the growth and development of the cells. The transformation of information from one generation to another is called heredity and the genes which are the fundamental part of the chromosome are responsible for the transformation. The chromosomes are present in pairs. Each pair is responsible for a specific part or function in the cell. These chromosomes are distributed equally in new cells during mitosis. Mitosis is thus described a s the chromosomal division. This chromosomal division is accompanied by the cytoplasmic division called as cytokinesis. The mitosis along with cytokinesis results in the formation of two daughter cells. This is also called as cell cycle. Cell cycle is the period in which the cell divides and becomes two daughter cells. ... There are four stages in mitosis. They are prophase, metaphase, anaphase and telophase. (Allen and Harper 2011). Prophase is the first stage of mitosis which is visible. The chromosomes, which are randomly distributed in the cytoplasm condense to form the distinct part. They are held in a single line. Metaphase is the stage in which the chromosome is attached to the centromere through the spindle fibril. The centromeres are connected to the centrioles and the centrioles pulls the chromatids to the opposite directions. This step initiates the cell division. Anaphase is the stage where the chromosomes move to the position of centriole. The chromosomes are seen as complete sets in the opposite side of the cell. Telophase is the last stage of mitosis in which the chromosomes begins to loosen itself from the thicker rigid structure into thin filaments. The division of the cytoplasm starts at this particular point. Materials required: Prepared slide of Allium root tip Prepared slide of whi te fish blastula. Microscope. Method: 1. The prepared slide of allium (onion) root tip was mounted on the microscope. 2. The 4x objective lenswas used to focus the centre of the slide. 3. The cells undergoing the four stages of mitosis such as prophase, metaphase , anaphase and telophase were identified in the slide using the 10x magnification. 4. Using the 40x magnification in the microscope, the distinct chromosomes and the other cell structures were identified. 5. Similarly, the whitefish blastula slide was mounted on the microscope and the blastula sections were observed using the 4x magnification. 6. Then 40 x magnification was chosen to examine the chromosomes. The cells in each phase of mitosis were identified. Result: For the Onion Root tip: 1. In the interphase stage, nucleus,